Detailed information of TA system TA029039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1147312..1147930
• Replicon: chromosome
• Accession: NC_020528
• Organism: Sinorhizobium meliloti 2011

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: SM2011_RS19675
• Protein ID: WP_010969011.1
• Coordinates: 1147634..1147930 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SM2011_RS19670
• Protein ID: WP_010969010.1
• Coordinates: 1147312..1147620 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM2011_RS19625	1142391..1142711	-	321	WP_003527320.1	YnfA family protein	-
SM2011_RS36235	1142786..1142959	-	174	WP_010969006.1	hypothetical protein	-
SM2011_RS36240	1142973..1143146	-	174	WP_003527318.1	hypothetical protein	-
SM2011_RS19640	1143382..1144083	+	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
SM2011_RS19645	1144307..1144648	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
SM2011_RS19650	1145233..1145499	+	267	WP_003527312.1	sel1 repeat family protein	-
SM2011_RS19655	1145521..1146642	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
SM2011_RS19665	1147071..1147301	-	231	WP_026029805.1	hypothetical protein	-
SM2011_RS19670	1147312..1147620	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
SM2011_RS19675	1147634..1147930	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SM2011_RS19680	1147978..1148283	-	306	WP_003527305.1	hypothetical protein	-
SM2011_RS19685	1148611..1149648	+	1038	WP_003527304.1	porin	-
SM2011_RS19690	1149758..1150825	-	1068	WP_014526650.1	site-specific integrase	-
SM2011_RS34490	1150952..1151898	+	947	WP_011970797.1	IS630-like element ISRm2011-2 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T29039 WP_010969011.1 NC_020528:c1147930-1147634 [Sinorhizobium meliloti 2011]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T29039 NC_020528:c1147930-1147634 [Sinorhizobium meliloti 2011]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT29039 WP_010969010.1 NC_020528:c1147620-1147312 [Sinorhizobium meliloti 2011]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT29039 NC_020528:c1147620-1147312 [Sinorhizobium meliloti 2011]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61