Detailed information of TA system TA290265

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73071..73400
• Replicon: plasmid 2
• Accession: NZ_LR890199
• Organism: Escherichia coli isolate MINF_1D-sc-2280456

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JMV89_RS24695
• Protein ID: WP_001312861.1
• Coordinates: 73071..73229 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	73273..73400 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMV89_RS24655	68183..68410	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
JMV89_RS24660	68498..69175	-	678	WP_001348626.1	PAS domain-containing protein	-
JMV89_RS24665	69309..69692	-	384	WP_001151566.1	relaxosome protein TraM	-
JMV89_RS24670	70023..70625	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
JMV89_RS24675	70922..71743	-	822	WP_001234469.1	DUF945 domain-containing protein	-
JMV89_RS24680	71862..72149	-	288	WP_000107535.1	hypothetical protein	-
JMV89_RS24685	72174..72380	-	207	WP_000275859.1	hypothetical protein	-
JMV89_RS24690	72293..72628	-	336	WP_013023876.1	hypothetical protein	-
JMV89_RS24695	73071..73229	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	73273..73400	-	128	NuclAT_0	-	Antitoxin
-	73273..73400	-	128	NuclAT_0	-	Antitoxin
-	73273..73400	-	128	NuclAT_0	-	Antitoxin
-	73273..73400	-	128	NuclAT_0	-	Antitoxin
-	74842..74944	-	103	NuclAT_1	-	-
-	74842..74944	-	103	NuclAT_1	-	-
-	74842..74944	-	103	NuclAT_1	-	-
-	74842..74944	-	103	NuclAT_1	-	-
JMV89_RS24705	74956..75675	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
JMV89_RS24710	75672..76106	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
JMV89_RS24715	76161..78119	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / blaCTX-M-15 / sitABCD	iucA / iucB / iucC / iucD / iutA	1..125802	125802

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T290265 WP_001312861.1 NZ_LR890199:c73229-73071 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 128 bp

>AT290265 NZ_LR890199:c73400-73273 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin