Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3915838..3916532 Replicon chromosome
Accession NZ_LR890198
Organism Escherichia coli isolate MINF_1D-sc-2280456

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag JMV89_RS18780 Protein ID WP_001263501.1
Coordinates 3915838..3916236 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JMV89_RS18785 Protein ID WP_000554755.1
Coordinates 3916239..3916532 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV89_RS18755 3911203..3911661 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
JMV89_RS18760 3911922..3913379 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
JMV89_RS18765 3913436..3914050 - 615 WP_000602123.1 peptide chain release factor H -
JMV89_RS18770 3914047..3915186 - 1140 WP_201520084.1 RNA ligase RtcB family protein -
JMV89_RS18775 3915376..3915828 - 453 WP_001059897.1 GNAT family N-acetyltransferase -
JMV89_RS18780 3915838..3916236 - 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JMV89_RS18785 3916239..3916532 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JMV89_RS18790 3916584..3917639 - 1056 WP_001226170.1 DNA polymerase IV -
JMV89_RS18795 3917710..3918495 - 786 WP_000207543.1 putative lateral flagellar export/assembly protein LafU -
JMV89_RS18800 3918467..3920179 + 1713 Protein_3687 flagellar biosynthesis protein FlhA -
JMV89_RS18805 3920207..3920701 + 495 WP_139174989.1 hypothetical protein -
JMV89_RS18810 3920696..3921193 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T290256 WP_001263501.1 NZ_LR890198:c3916236-3915838 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT290256 WP_000554755.1 NZ_LR890198:c3916532-3916239 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References