29024

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 721215..722458 Replicon
Accession NC_020521
Organism Legionella pneumophila subsp. pneumophila str. Philadelphia 1

Toxin (Protein)


Gene name HipT Uniprot ID Q5ZSZ6
Locus tag LPE509_RS03260 Protein ID WP_010948074.1
Coordinates 721215..722153 (-) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5ZSZ7
Locus tag LPE509_RS03265 Protein ID WP_010948073.1
Coordinates 722150..722458 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPE509_RS03235 716685..717350 + 666 WP_015444114.1 hypothetical protein -
LPE509_RS03240 717350..717875 + 526 Protein_640 hypothetical protein -
LPE509_RS03245 718091..719359 - 1269 WP_010948076.1 lpg2372 family Dot/Icm T4SS effector -
LPE509_RS03250 719696..719902 + 207 WP_015444115.1 hypothetical protein -
LPE509_RS03255 720058..720672 - 615 WP_010948075.1 hypothetical protein -
LPE509_RS03260 721215..722153 - 939 WP_010948074.1 lpg2370 family Dot/Icm T4SS effector Toxin
LPE509_RS03265 722150..722458 - 309 WP_010948073.1 HipA N-terminal domain-containing protein Antitoxin
LPE509_RS03270 722455..722682 - 228 WP_006870829.1 helix-turn-helix transcriptional regulator -
LPE509_RS03275 723515..723790 + 276 WP_010948072.1 helix-turn-helix domain-containing protein -
LPE509_RS03280 723856..725607 + 1752 WP_010948071.1 DUF927 domain-containing protein -
LPE509_RS16315 725610..725750 + 141 WP_153802426.1 hypothetical protein -
LPE509_RS03285 725935..726300 + 366 WP_010948070.1 TraK family protein -
LPE509_RS03290 726657..727022 - 366 WP_010948069.1 BRCT domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 727261..728436 1175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-276)

Antitoxin

HipA, N-terminal subdomain 1

(5-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36077.81 Da        Isoelectric Point: 8.6882

>T29024 WP_010948074.1 NC_020521:c722153-721215 [Legionella pneumophila subsp. pneumophila LPE509]
MKHCPITYEKISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSVQGVQKKLSAKLKIKEGCFEIVDQYG
QYILKPQSDIYPELPENEAITMTLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLLNSTIAQKNTKEELALPLKG
KKNNLTKSDFLKYFAIEKLGLNQNVIDGIVQEFHQVIPKWQELIGFSFLSQEMQEKYLELLEQRCKRLNFFD

Download         Length: 939 bp

>T29024 NC_020521:c722153-721215 [Legionella pneumophila subsp. pneumophila LPE509]
ATGAAACACTGCCCTATTACTTATGAAAAGATTAGTGACCAGGAAAACTATTCGCAACGTGGATTGCATTTGCTTTCTCC
TCAATTAAAAAACCTTAGTCCATTGGATTTAAGTGCTGATGAGCAGCGTCAGGAAGCAATTGCTCGAGTAGGAAAGATGT
CTGTTCAAGGCGTCCAAAAGAAACTAAGCGCAAAACTAAAGATTAAAGAAGGCTGTTTTGAAATCGTGGATCAATATGGT
CAGTATATTTTAAAACCACAAAGTGATATTTATCCAGAATTGCCTGAAAATGAAGCCATTACTATGACCCTTGCAAAAAC
TATAGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTAACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACAAAATATAAAAGT
TCTATGGAAAAAGTAATTGCAGTCATAGAACAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGTTGAC
GTTGTTTAACTTCTTGGTCGGTAATGAAGACATGCATCTAAAAAACTTTTCCTTAATTACGAAGGATAGAAAAATTTCCA
TATCACCAGCCTACGATTTACTTAACTCAACCATCGCTCAAAAAAACACAAAAGAAGAACTGGCTTTACCTCTAAAAGGA
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCATTGAAAAATTAGGATTAAACCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAGGTAATACCAAAATGGCAAGAGCTGATTGGCTTCAGTTTTTTATCTCAAGAGATGC
AGGAAAAATATCTCGAATTGCTAGAGCAACGATGCAAGCGATTAAATTTTTTTGATTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11557.38 Da        Isoelectric Point: 4.5994

>AT29024 WP_010948073.1 NC_020521:c722458-722150 [Legionella pneumophila subsp. pneumophila LPE509]
MRKAYVSVSGIKAGILEELQGGTYQFTYFEDYHGAPVSLTMPLKNKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLILVGQDVVGAVTIEEIR

Download         Length: 309 bp

>AT29024 NC_020521:c722458-722150 [Legionella pneumophila subsp. pneumophila LPE509]
ATGAGAAAAGCATACGTATCGGTGAGTGGCATTAAGGCTGGAATATTAGAAGAATTACAAGGTGGAACCTATCAATTTAC
CTACTTTGAGGATTATCACGGCGCACCTGTTTCACTCACTATGCCGTTGAAAAATAAGGTCTATGATTTTGACGTGTTCC
CCCCTTTTTTTGAAGGATTACTTCCTGAAGGCATTATGCTTGAAGCACTATTACGCAAATACAAGATAGATAAAAATGAT
TATTTTGGTCAATTGATACTAGTTGGCCAAGATGTCGTAGGCGCAGTGACTATTGAGGAAATAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VKC
PDB 7VKB
PDB 7WCF

Antitoxin

Source ID Structure
AlphaFold DB Q5ZSZ7

References