Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1948388..1948929 Replicon chromosome
Accession NZ_LR883965
Organism Escherichia coli isolate 2016-17-550

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JMV10_RS09390 Protein ID WP_000615976.1
Coordinates 1948651..1948929 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JMV10_RS09385 Protein ID WP_000729704.1
Coordinates 1948388..1948648 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV10_RS09360 1943424..1944563 + 1140 WP_001402040.1 flagellar type III secretion system protein FlhB -
JMV10_RS09365 1944547..1946640 + 2094 WP_021499017.1 flagellar biosynthesis protein FlhA -
JMV10_RS09370 1946745..1947023 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JMV10_RS09375 1947016..1947372 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JMV10_RS09380 1947429..1948202 - 774 WP_000543899.1 C40 family peptidase -
JMV10_RS09385 1948388..1948648 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JMV10_RS09390 1948651..1948929 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JMV10_RS09395 1949085..1949825 + 741 WP_001225679.1 murein L,D-transpeptidase -
JMV10_RS09400 1949796..1950563 - 768 WP_000333380.1 class II glutamine amidotransferase -
JMV10_RS09405 1950769..1951347 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T290174 WP_000615976.1 NZ_LR883965:1948651-1948929 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT290174 WP_000729704.1 NZ_LR883965:1948388-1948648 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References