Detailed information of TA system TA290142

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61251..61668
• Replicon: plasmid 3
• Accession: NZ_LR883014
• Organism: Escherichia coli isolate L4_E1441_ETEC

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLZ95_RS24645
• Protein ID: WP_001312861.1
• Coordinates: 61251..61409 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	61474..61668 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLZ95_RS24615	56764..57111	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
JLZ95_RS24620	57111..57788	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
JLZ95_RS24625	57841..58032	-	192	Protein_58	hypothetical protein	-
JLZ95_RS24630	58342..59019	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
JLZ95_RS24635	59019..59366	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
JLZ95_RS24640	59386..60957	+	1572	WP_099554548.1	IS66 family transposase	-
JLZ95_RS24645	61251..61409	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	61474..61668	-	195	NuclAT_0	-	Antitoxin
-	61474..61668	-	195	NuclAT_0	-	Antitoxin
-	61474..61668	-	195	NuclAT_0	-	Antitoxin
-	61474..61668	-	195	NuclAT_0	-	Antitoxin
JLZ95_RS24650	61680..62399	-	720	WP_001734594.1	plasmid SOS inhibition protein A	-
JLZ95_RS24655	62396..62830	-	435	WP_000845954.1	conjugation system SOS inhibitor PsiB	-
JLZ95_RS24660	62885..64848	-	1964	Protein_65	ParB/RepB/Spo0J family partition protein	-
JLZ95_RS24665	64910..65470	+	561	Protein_66	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	eltA / eltB / espC / cssA	1..94840	94840
	-	inside	IScluster/Tn	-	espC / cssA	41145..71609	30464

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T290142 WP_001312861.1 NZ_LR883014:c61409-61251 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 195 bp

>AT290142 NZ_LR883014:c61668-61474 [Escherichia coli]
TCACGCGGATTTCCCGTAGCCTGAATGAGCGTATTCTTTTCAGGAAAAGTGAATGTGGCCAGCGTGCAGGGATATGAGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin