Detailed information of TA system TA029014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/-
• Location: 2648100..2648793
• Replicon: chromosome
• Accession: NC_020518
• Organism: Escherichia coli str. K-12 substr. MDS42

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: ECMDS42_RS12785
• Protein ID: WP_000415584.1
• Coordinates: 2648497..2648793 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: ECMDS42_RS12780
• Protein ID: WP_000650107.1
• Coordinates: 2648100..2648495 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECMDS42_RS12770 (ECMDS42_2494)	2643964..2646222	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
ECMDS42_RS12775 (ECMDS42_2495)	2646360..2647967	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
ECMDS42_RS12780 (ECMDS42_2496)	2648100..2648495	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
ECMDS42_RS12785 (ECMDS42_2497)	2648497..2648793	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
ECMDS42_RS12790 (ECMDS42_2498)	2648998..2649480	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
ECMDS42_RS12795 (ECMDS42_2499)	2649533..2649925	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
ECMDS42_RS12800 (ECMDS42_2500)	2650077..2650736	+	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	-
ECMDS42_RS12805 (ECMDS42_2501)	2650733..2652082	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
ECMDS42_RS12810 (ECMDS42_2502)	2652128..2652460	-	333	WP_000917684.1	DUF2645 family protein	-
ECMDS42_RS12815 (ECMDS42_2503)	2652779..2653360	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
ECMDS42_RS12820 (ECMDS42_2504)	2653391..2653705	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T29014 WP_000415584.1 NC_020518:c2648793-2648497 [Escherichia coli str. K-12 substr. MDS42]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T29014 NC_020518:c2648793-2648497 [Escherichia coli str. K-12 substr. MDS42]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT29014 WP_000650107.1 NC_020518:c2648495-2648100 [Escherichia coli str. K-12 substr. MDS42]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT29014 NC_020518:c2648495-2648100 [Escherichia coli str. K-12 substr. MDS42]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65