Detailed information of TA system TA290138

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93333..93746
• Replicon: plasmid 2
• Accession: NZ_LR883013
• Organism: Escherichia coli isolate L4_E1441_ETEC

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: JLZ95_RS24105
• Protein ID: WP_000775238.1
• Coordinates: 93333..93494 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	93546..93746 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLZ95_RS24080	88705..89394	-	690	WP_000283390.1	conjugal transfer transcriptional regulator TraJ	-
JLZ95_RS24085	89581..89964	-	384	WP_001151524.1	relaxosome protein TraM	-
JLZ95_RS24090	90286..90888	+	603	WP_077631932.1	transglycosylase SLT domain-containing protein	-
JLZ95_RS24095	91185..92003	-	819	WP_074481281.1	DUF945 domain-containing protein	-
JLZ95_RS24100	92114..92410	-	297	WP_001272248.1	hypothetical protein	-
JLZ95_RS24105	93333..93494	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	93546..93746	-	201	NuclAT_0	-	Antitoxin
-	93546..93746	-	201	NuclAT_0	-	Antitoxin
-	93546..93746	-	201	NuclAT_0	-	Antitoxin
-	93546..93746	-	201	NuclAT_0	-	Antitoxin
JLZ95_RS24110	93703..94017	-	315	WP_000872086.1	hypothetical protein	-
JLZ95_RS24115	94014..94733	-	720	WP_001276220.1	plasmid SOS inhibition protein A	-
JLZ95_RS24120	94730..95164	-	435	WP_000845955.1	conjugation system SOS inhibitor PsiB	-
JLZ95_RS24125	95219..97183	-	1965	WP_074481283.1	ParB/RepB/Spo0J family partition protein	-
JLZ95_RS24130	97244..97477	-	234	WP_000005975.1	DUF905 domain-containing protein	-
JLZ95_RS24135	97534..98061	-	528	WP_074481284.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA15 / ant(3'')-Ia / qacE / sul1 / tet(A)	cofA	1..130302	130302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T290138 WP_000775238.1 NZ_LR883013:c93494-93333 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

Antitoxin

Nucleotide

• Length: 201 bp

>AT290138 NZ_LR883013:c93746-93546 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGTGCAGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACAGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCTA

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin