Detailed information of TA system TA290087

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 336692..337104
• Replicon: plasmid 2
• Accession: NZ_LR882998
• Organism: Escherichia coli isolate L3_E36_ETEC

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A2H4TK50
• Locus tag: JL022_RS27055
• Protein ID: WP_000775237.1
• Coordinates: 336943..337104 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	336692..336897 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL022_RS27025	332374..332901	+	528	WP_000290790.1	single-stranded DNA-binding protein	-
JL022_RS27030	332958..333191	+	234	WP_000005971.1	DUF905 domain-containing protein	-
JL022_RS27035	333255..335219	+	1965	WP_001759171.1	ParB/RepB/Spo0J family partition protein	-
JL022_RS27040	335274..335708	+	435	WP_000845950.1	conjugation system SOS inhibitor PsiB	-
JL022_RS27045	335705..336424	+	720	WP_099481790.1	plasmid SOS inhibition protein A	-
JL022_RS27050	336421..336735	+	315	WP_000872086.1	hypothetical protein	-
-	336692..336897	+	206	NuclAT_2	-	Antitoxin
-	336692..336897	+	206	NuclAT_2	-	Antitoxin
-	336692..336897	+	206	NuclAT_2	-	Antitoxin
-	336692..336897	+	206	NuclAT_2	-	Antitoxin
JL022_RS27055	336943..337104	+	162	WP_000775237.1	type I toxin-antitoxin system Hok family toxin	Toxin
JL022_RS27060	337342..337720	-	379	Protein_406	hypothetical protein	-
JL022_RS27065	338016..338339	+	324	WP_000533253.1	hypothetical protein	-
JL022_RS27070	338398..338640	+	243	WP_000540588.1	hypothetical protein	-
JL022_RS27075	338906..339154	+	249	WP_071606928.1	hypothetical protein	-
JL022_RS27080	339622..339918	+	297	WP_001272251.1	hypothetical protein	-
JL022_RS27085	340029..340850	+	822	WP_001234441.1	DUF945 domain-containing protein	-
JL022_RS27090	341147..341737	-	591	WP_126728383.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	eltB / eltA / cofA / etpA / etpB / cfaA / cfaB / cfaC / cfaE / cfaD'	1..381858	381858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5946.16 Da
• Isoelectric Point: 8.5101

>T290087 WP_000775237.1 NZ_LR882998:336943-337104 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESGGK

Nucleotide

• Length: 162 bp

Antitoxin

Nucleotide

• Length: 206 bp

>AT290087 NZ_LR882998:336692-336897 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAATGTGGTCAGCGTGCTGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGACAAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A2H4TK50

Antitoxin