Detailed information of TA system TA290078

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45927..46339
• Replicon: plasmid 2
• Accession: NZ_LR882998
• Organism: Escherichia coli isolate L3_E36_ETEC

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A2H4TK50
• Locus tag: JL022_RS25350
• Protein ID: WP_000775237.1
• Coordinates: 46178..46339 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	45927..46132 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL022_RS25320	41609..42136	+	528	WP_000290790.1	single-stranded DNA-binding protein	-
JL022_RS25325	42193..42426	+	234	WP_000005971.1	DUF905 domain-containing protein	-
JL022_RS25330	42490..44454	+	1965	WP_001759171.1	ParB/RepB/Spo0J family partition protein	-
JL022_RS25335	44509..44943	+	435	WP_000845950.1	conjugation system SOS inhibitor PsiB	-
JL022_RS25340	44940..45659	+	720	WP_099481790.1	plasmid SOS inhibition protein A	-
JL022_RS25345	45656..45970	+	315	WP_000872086.1	hypothetical protein	-
-	45927..46132	+	206	NuclAT_1	-	Antitoxin
-	45927..46132	+	206	NuclAT_1	-	Antitoxin
-	45927..46132	+	206	NuclAT_1	-	Antitoxin
-	45927..46132	+	206	NuclAT_1	-	Antitoxin
JL022_RS25350	46178..46339	+	162	WP_000775237.1	type I toxin-antitoxin system Hok family toxin	Toxin
JL022_RS25355	46577..46955	-	379	Protein_65	hypothetical protein	-
JL022_RS25360	47251..47574	+	324	WP_000533253.1	hypothetical protein	-
JL022_RS25365	47633..47875	+	243	WP_000540588.1	hypothetical protein	-
JL022_RS25370	48141..48389	+	249	WP_071606928.1	hypothetical protein	-
JL022_RS25375	48857..49153	+	297	WP_001272251.1	hypothetical protein	-
JL022_RS25380	49264..50085	+	822	WP_001234441.1	DUF945 domain-containing protein	-
JL022_RS25385	50382..50972	-	591	WP_126728383.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	eltB / eltA / cofA / etpA / etpB / cfaA / cfaB / cfaC / cfaE / cfaD'	1..381858	381858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5946.16 Da
• Isoelectric Point: 8.5101

>T290078 WP_000775237.1 NZ_LR882998:46178-46339 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESGGK

Nucleotide

• Length: 162 bp

Antitoxin

Nucleotide

• Length: 206 bp

>AT290078 NZ_LR882998:45927-46132 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAATGTGGTCAGCGTGCTGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGACAAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A2H4TK50

Antitoxin