Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4950366..4950968 Replicon chromosome
Accession NZ_LR882997
Organism Escherichia coli isolate L3_E36_ETEC

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag JL022_RS24075 Protein ID WP_000897305.1
Coordinates 4950657..4950968 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JL022_RS24070 Protein ID WP_000356397.1
Coordinates 4950366..4950656 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL022_RS24045 4946292..4947194 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
JL022_RS24050 4947191..4947826 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
JL022_RS24055 4947823..4948752 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
JL022_RS24060 4949082..4949324 - 243 WP_001086388.1 hypothetical protein -
JL022_RS24065 4949543..4949761 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
JL022_RS24070 4950366..4950656 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
JL022_RS24075 4950657..4950968 - 312 WP_000897305.1 hypothetical protein Toxin
JL022_RS24080 4951197..4952105 + 909 WP_001162704.1 alpha/beta hydrolase -
JL022_RS24085 4952169..4953110 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
JL022_RS24090 4953155..4953592 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
JL022_RS24095 4953589..4954461 - 873 WP_000920762.1 virulence factor BrkB family protein -
JL022_RS24100 4954455..4955054 - 600 WP_001315111.1 glucose-1-phosphatase -
JL022_RS24105 4955153..4955938 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T290077 WP_000897305.1 NZ_LR882997:c4950968-4950657 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT290077 WP_000356397.1 NZ_LR882997:c4950656-4950366 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References