Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 646123..646922 Replicon chromosome
Accession NZ_LR882997
Organism Escherichia coli isolate L3_E36_ETEC

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag JL022_RS03140 Protein ID WP_000347267.1
Coordinates 646123..646587 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JL022_RS03145 Protein ID WP_001307405.1
Coordinates 646587..646922 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL022_RS03110 641124..641558 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
JL022_RS03115 641576..642454 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JL022_RS03120 642444..643223 - 780 WP_001697011.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JL022_RS03125 643234..643707 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JL022_RS03130 643730..645010 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
JL022_RS03135 645259..646068 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JL022_RS03140 646123..646587 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JL022_RS03145 646587..646922 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JL022_RS03150 647071..648642 - 1572 WP_001273741.1 galactarate dehydratase -
JL022_RS03155 649017..650351 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JL022_RS03160 650367..651137 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 646123..657578 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T290060 WP_000347267.1 NZ_LR882997:c646587-646123 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT290060 WP_001307405.1 NZ_LR882997:c646922-646587 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References