Detailed information of TA system TA290012

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23258..23675
• Replicon: plasmid 2
• Accession: NZ_LR882974
• Organism: Escherichia coli isolate L2_E1649

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLZ81_RS23060
• Protein ID: WP_001312861.1
• Coordinates: 23258..23416 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	23481..23675 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLZ81_RS23030	18482..18892	-	411	Protein_18	Tn3 family transposase	-
JLZ81_RS23035	19101..19247	+	147	Protein_19	IS30 family transposase	-
JLZ81_RS23040	19583..19966	-	384	WP_200986833.1	hypothetical protein	-
JLZ81_RS23045	20565..21164	-	600	WP_032302271.1	proQ/FINO family protein	-
JLZ81_RS23050	21378..21563	+	186	Protein_22	transposase domain-containing protein	-
JLZ81_RS23060	23258..23416	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	23481..23675	-	195	NuclAT_0	-	Antitoxin
-	23481..23675	-	195	NuclAT_0	-	Antitoxin
-	23481..23675	-	195	NuclAT_0	-	Antitoxin
-	23481..23675	-	195	NuclAT_0	-	Antitoxin
JLZ81_RS23065	23687..24406	-	720	WP_001276221.1	plasmid SOS inhibition protein A	-
JLZ81_RS23070	24403..24837	-	435	WP_000845924.1	conjugation system SOS inhibitor PsiB	-
JLZ81_RS23075	24892..26190	-	1299	Protein_27	hypothetical protein	-
JLZ81_RS23080	26189..27025	+	837	Protein_28	IS91 family transposase	-
JLZ81_RS23085	27302..27667	+	366	WP_000124102.1	hypothetical protein	-
JLZ81_RS23090	27652..27795	+	144	Protein_30	IS91 family transposase	-
JLZ81_RS23095	27982..28595	-	614	Protein_31	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..86517	86517
	-	inside	IScluster/Tn	-	-	17051..30778	13727

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T290012 WP_001312861.1 NZ_LR882974:c23416-23258 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 195 bp

>AT290012 NZ_LR882974:c23675-23481 [Escherichia coli]
TCACGCGGATTTCCCGTAGCCTGAATGAGCGTATTCTTTTCAGGAAAAGTGAATGTGGCCAGCGCGCAGGGATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACAGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin