Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2267144..2268063 Replicon chromosome
Accession NZ_LR882500
Organism Mycobacterium tuberculosis variant microti strain 94-2272 isolate human

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JOA04_RS10475 Protein ID WP_003900449.1
Coordinates 2267458..2268063 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JOA04_RS10470 Protein ID WP_003410124.1
Coordinates 2267144..2267449 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JOA04_RS10440 2262155..2263411 - 1257 WP_003410095.1 HNH endonuclease -
JOA04_RS10445 2263765..2264340 + 576 WP_003410100.1 hypothetical protein -
JOA04_RS10450 2264337..2265377 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JOA04_RS10455 2265619..2266338 + 720 WP_003410108.1 DUF433 domain-containing protein -
JOA04_RS10460 2266328..2266744 + 417 WP_003410114.1 hypothetical protein -
JOA04_RS10465 2266760..2267059 - 300 WP_003410120.1 hypothetical protein -
JOA04_RS10470 2267144..2267449 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JOA04_RS10475 2267458..2268063 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JOA04_RS10480 2268088..2268447 - 360 WP_003410131.1 hypothetical protein -
JOA04_RS10485 2268607..2269284 - 678 WP_003900450.1 hypothetical protein -
JOA04_RS10490 2269236..2269472 - 237 WP_003410133.1 hypothetical protein -
JOA04_RS10495 2269679..2270575 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T289937 WP_003900449.1 NZ_LR882500:c2268063-2267458 [Mycobacterium tuberculosis variant microti]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT289937 WP_003410124.1 NZ_LR882500:c2267449-2267144 [Mycobacterium tuberculosis variant microti]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References