Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2215291..2216159 Replicon chromosome
Accession NZ_LR882500
Organism Mycobacterium tuberculosis variant microti strain 94-2272 isolate human

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag JOA04_RS10170 Protein ID WP_010886136.1
Coordinates 2215291..2215668 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag JOA04_RS10175 Protein ID WP_003409886.1
Coordinates 2215710..2216159 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JOA04_RS10120 2211080..2211532 - 453 WP_003899095.1 lipoprotein -
JOA04_RS10125 2211596..2211997 + 402 WP_003409869.1 hypothetical protein -
JOA04_RS10130 2211990..2212172 - 183 WP_003409870.1 hypothetical protein -
JOA04_RS10135 2212286..2212636 - 351 WP_003409871.1 hypothetical protein -
JOA04_RS10140 2212647..2213549 - 903 WP_003409874.1 hypothetical protein -
JOA04_RS10145 2213570..2213761 - 192 WP_003409876.1 hypothetical protein -
JOA04_RS10150 2213762..2214058 - 297 WP_003409877.1 hypothetical protein -
JOA04_RS10155 2214298..2214513 + 216 WP_003409878.1 antitoxin -
JOA04_RS10160 2214510..2214821 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
JOA04_RS10165 2214795..2215316 - 522 WP_003904745.1 hypothetical protein -
JOA04_RS10170 2215291..2215668 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
JOA04_RS10175 2215710..2216159 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JOA04_RS10180 2216156..2216701 + 546 WP_003409891.1 SecB-like chaperone -
JOA04_RS10185 2216590..2217204 - 615 WP_003901296.1 hypothetical protein -
JOA04_RS10190 2217253..2217549 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
JOA04_RS10195 2217546..2217797 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
JOA04_RS10200 2217784..2218278 + 495 WP_003899099.1 hypothetical protein -
JOA04_RS10205 2218438..2218845 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
JOA04_RS10210 2218849..2219121 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JOA04_RS10215 2219154..2220374 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T289931 WP_010886136.1 NZ_LR882500:2215291-2215668 [Mycobacterium tuberculosis variant microti]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT289931 WP_003409886.1 NZ_LR882500:2215710-2216159 [Mycobacterium tuberculosis variant microti]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References