Detailed information of TA system TA028970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 280583..281150
• Replicon: chromosome
• Accession: NC_020300
• Organism: Bartonella australis Aust/NH1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M1P2U3
• Locus tag: BANH1_RS01330
• Protein ID: WP_015397649.1
• Coordinates: 280872..281150 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: BANH1_RS01325
• Protein ID: WP_015397648.1
• Coordinates: 280583..280861 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BANH1_RS01295	275853..276359	+	507	WP_015397642.1	phage major tail tube protein	-
BANH1_RS01300	276362..276649	+	288	WP_015397643.1	phage tail assembly protein	-
BANH1_RS07300	276646..276759	+	114	WP_144050554.1	GpE family phage tail protein	-
BANH1_RS01305	276756..278639	+	1884	WP_015397644.1	phage tail protein	-
BANH1_RS01310	278645..279022	+	378	WP_015397645.1	phage tail protein	-
BANH1_RS01315	279022..279246	+	225	WP_015397646.1	tail protein X	-
BANH1_RS01320	279243..280547	+	1305	WP_015397647.1	phage late control protein D	-
BANH1_RS01325	280583..280861	-	279	WP_015397648.1	HigA family addiction module antidote protein	Antitoxin
BANH1_RS01330	280872..281150	-	279	WP_015397649.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BANH1_RS01335	281245..281922	+	678	WP_015397650.1	lysozyme	-
BANH1_RS01340	281922..282140	+	219	WP_015397651.1	hypothetical protein	-
BANH1_RS01350	282367..283671	+	1305	WP_015397652.1	class I SAM-dependent methyltransferase	-
BANH1_RS01355	283788..284543	+	756	WP_015397653.1	phage antirepressor KilAC domain-containing protein	-
BANH1_RS01360	284845..285057	+	213	WP_015397654.1	helix-turn-helix transcriptional regulator	-
BANH1_RS01365	285175..285681	-	507	WP_015397655.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	260224..287904	27680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10875.46 Da
• Isoelectric Point: 9.3599

>T28970 WP_015397649.1 NC_020300:c281150-280872 [Bartonella australis Aust/NH1]
MIISFRHKGLREFYETGSTKGIQIKHAQKLRYILAILDVAEDVNDVAFPSFRLHSLQGDLKNYWSIRVNGNWRVTFKFIG
KDIELVDYQDYH

Nucleotide

• Length: 279 bp

>T28970 NC_020300:c281150-280872 [Bartonella australis Aust/NH1]
ATGATTATTAGCTTTAGACACAAAGGATTGAGGGAGTTTTATGAAACTGGCTCAACGAAAGGTATCCAAATAAAGCACGC
TCAAAAGCTTCGATATATTCTGGCAATCCTCGATGTAGCTGAAGATGTGAACGATGTAGCGTTCCCGAGCTTCCGTCTGC
ATTCCCTTCAAGGTGATTTAAAAAATTATTGGTCAATCCGTGTAAATGGGAACTGGCGCGTTACATTTAAGTTTATAGGC
AAAGATATTGAGCTCGTTGATTATCAAGATTATCATTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10329.01 Da
• Isoelectric Point: 10.2028

>AT28970 WP_015397648.1 NC_020300:c280861-280583 [Bartonella australis Aust/NH1]
MMHNPPHPGYILRDEVIEELGLTVSEAAQRLAMSRVALSRIINGHASISTDLAIRLEMAGISTARLWLSLQTSFDLWQAK
QRKQPHITRLVA

Nucleotide

• Length: 279 bp

>AT28970 NC_020300:c280861-280583 [Bartonella australis Aust/NH1]
ATGATGCACAATCCTCCTCACCCTGGTTATATCTTACGTGATGAAGTGATCGAGGAACTTGGTTTAACAGTGAGTGAAGC
AGCACAGCGCTTAGCAATGTCGCGTGTAGCCTTATCACGGATCATCAATGGGCATGCAAGCATTTCAACTGACCTCGCTA
TCCGCCTTGAAATGGCCGGTATTAGCACCGCACGCTTGTGGCTTTCACTGCAAACAAGCTTCGACCTTTGGCAAGCAAAG
CAGCGCAAACAGCCCCATATAACACGTTTAGTAGCTTAG

Structures

Toxin

• AlphaFold DB: M1P2U3