Detailed information of TA system TA289655

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 71652..72078
• Replicon: plasmid 2
• Accession: NZ_LR882494
• Organism: Escherichia coli isolate 2016-17-292

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IHO92_RS23950
• Protein ID: WP_001312861.1
• Coordinates: 71652..71810 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	71854..72078 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHO92_RS23925	67026..67715	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
IHO92_RS23930	67902..68285	-	384	WP_001151524.1	relaxosome protein TraM	-
IHO92_RS23935	68606..69208	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
IHO92_RS23940	69505..70326	-	822	WP_001234426.1	DUF945 domain-containing protein	-
IHO92_RS23945	70444..70731	-	288	WP_000107535.1	hypothetical protein	-
IHO92_RS23950	71652..71810	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	71854..72078	-	225	NuclAT_0	-	Antitoxin
-	71854..72078	-	225	NuclAT_0	-	Antitoxin
-	71854..72078	-	225	NuclAT_0	-	Antitoxin
-	71854..72078	-	225	NuclAT_0	-	Antitoxin
IHO92_RS23955	71890..72078	+	189	WP_001299721.1	hypothetical protein	-
IHO92_RS23960	72090..72809	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
IHO92_RS23965	72806..73240	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
IHO92_RS23970	73295..73492	-	198	Protein_77	hypothetical protein	-
IHO92_RS23975	73520..73753	-	234	WP_000005990.1	DUF905 family protein	-
IHO92_RS23980	73821..74360	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
IHO92_RS23985	74386..74592	-	207	WP_000275856.1	hypothetical protein	-
IHO92_RS23990	75731..76702	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..97083	97083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T289655 WP_001312861.1 NZ_LR882494:c71810-71652 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT289655 NZ_LR882494:c72078-71854 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin