Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1066959..1067686 Replicon chromosome
Accession NZ_LR882493
Organism Escherichia coli isolate 2016-17-292

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag IHO92_RS05200 Protein ID WP_000547564.1
Coordinates 1066959..1067270 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IHO92_RS05205 Protein ID WP_000126294.1
Coordinates 1067267..1067686 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHO92_RS05170 1062101..1063810 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
IHO92_RS05175 1063820..1064362 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
IHO92_RS05180 1064362..1065129 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
IHO92_RS05185 1065126..1065536 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
IHO92_RS05190 1065529..1065999 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
IHO92_RS05195 1066024..1066785 + 762 WP_094316645.1 hypothetical protein -
IHO92_RS05200 1066959..1067270 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
IHO92_RS05205 1067267..1067686 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
IHO92_RS05210 1067800..1069224 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
IHO92_RS05215 1069233..1070690 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
IHO92_RS05220 1070950..1071960 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
IHO92_RS05225 1072109..1072636 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T289641 WP_000547564.1 NZ_LR882493:1066959-1067270 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT289641 WP_000126294.1 NZ_LR882493:1067267-1067686 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References