Detailed information of TA system TA028948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1864037..1864602
• Replicon: chromosome
• Accession: NC_020260
• Organism: Cronobacter sakazakii SP291

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CSSP291_RS08940
• Protein ID: WP_004388463.1
• Coordinates: 1864324..1864602 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: CSSP291_RS08935
• Protein ID: WP_004388462.1
• Coordinates: 1864037..1864324 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSSP291_RS08920	1859634..1860500	+	867	WP_004384960.1	ABC transporter permease	-
CSSP291_RS08925	1860530..1861588	-	1059	WP_004384961.1	YncE family protein	-
CSSP291_RS08930	1861866..1863983	+	2118	WP_015386728.1	TonB-dependent receptor	-
CSSP291_RS08935	1864037..1864324	-	288	WP_004388462.1	HigA family addiction module antidote protein	Antitoxin
CSSP291_RS08940	1864324..1864602	-	279	WP_004388463.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSSP291_RS08945	1864902..1866506	+	1605	WP_004388464.1	alpha-amylase family protein	-
CSSP291_RS08950	1866508..1867494	-	987	WP_007888785.1	SDR family oxidoreductase	-
CSSP291_RS08955	1867603..1869540	-	1938	WP_015386729.1	HAMP domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10559.03 Da
• Isoelectric Point: 10.0888

>T28948 WP_004388463.1 NC_020260:c1864602-1864324 [Cronobacter sakazakii SP291]
MIRSFRHKGLQRYFETGSTAGIHARHARKISLRLAVLNQAISPADVDLPGFFLHPLTGDRKGIWAVTVSGNWRITFEFRD
GDVFIVNYEDYH

Nucleotide

• Length: 279 bp

>T28948 NC_020260:c1864602-1864324 [Cronobacter sakazakii SP291]
ATGATCAGAAGCTTCAGGCATAAAGGCCTGCAACGCTATTTCGAAACGGGTTCCACCGCAGGCATTCATGCCCGCCATGC
CAGAAAGATTTCTCTGCGGCTGGCGGTGCTGAATCAGGCGATAAGCCCCGCTGACGTCGACTTACCGGGCTTTTTCCTGC
ATCCCCTGACGGGGGATCGTAAAGGTATATGGGCTGTGACCGTCAGCGGAAACTGGCGCATCACGTTTGAGTTTCGGGAT
GGCGATGTCTTTATCGTCAATTATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10428.03 Da
• Isoelectric Point: 8.7791

>AT28948 WP_004388462.1 NC_020260:c1864324-1864037 [Cronobacter sakazakii SP291]
MATMFNPPHPGRLVQESMEALGLSARALAKALDVAPSTVQRLLVGKSDVSPEMALRLSAVLGSSPHVWLGMQNEYDLFQA
RQSVDFSHLQRLQRA

Nucleotide

• Length: 288 bp

>AT28948 NC_020260:c1864324-1864037 [Cronobacter sakazakii SP291]
ATGGCAACGATGTTTAACCCACCGCACCCGGGCAGGCTGGTGCAGGAGTCAATGGAAGCGCTCGGCCTCAGCGCCCGTGC
GCTGGCCAAAGCGCTGGACGTCGCGCCGTCGACGGTGCAGCGGCTGCTGGTCGGCAAATCTGACGTCTCCCCGGAAATGG
CGCTGCGTCTCTCGGCAGTCCTTGGCAGCTCGCCGCATGTCTGGCTGGGTATGCAAAACGAATACGATCTCTTTCAGGCG
CGCCAGTCTGTTGATTTCTCGCACCTGCAACGGTTGCAGAGGGCATAA