Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1017176..1017903 Replicon chromosome
Accession NZ_LR878365
Organism Shigella flexneri 3a isolate 83

Toxin (Protein)


Gene name higB Uniprot ID A0A2Y4XNA6
Locus tag JMV05_RS05005 Protein ID WP_000547562.1
Coordinates 1017176..1017487 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JMV05_RS05010 Protein ID WP_000126288.1
Coordinates 1017484..1017903 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV05_RS04975 (1012334) 1012334..1014019 + 1686 WP_025758592.1 formate hydrogenlyase subunit HycE -
JMV05_RS04980 (1014029) 1014029..1014571 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
JMV05_RS04985 (1014571) 1014571..1015338 + 768 WP_025758590.1 formate hydrogenlyase subunit HycG -
JMV05_RS04990 (1015335) 1015335..1015745 + 411 WP_001291916.1 formate hydrogenlyase assembly protein HycH -
JMV05_RS04995 (1015738) 1015738..1016208 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
JMV05_RS05000 (1016260) 1016260..1017003 + 744 WP_024259998.1 hypothetical protein -
JMV05_RS05005 (1017176) 1017176..1017487 + 312 WP_000547562.1 type II toxin-antitoxin system HigB family toxin Toxin
JMV05_RS05010 (1017484) 1017484..1017903 + 420 WP_000126288.1 helix-turn-helix domain-containing protein Antitoxin
JMV05_RS05015 (1018018) 1018018..1019442 - 1425 WP_119182051.1 6-phospho-beta-glucosidase AscB -
JMV05_RS05020 (1019451) 1019451..1020908 - 1458 WP_001107865.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
JMV05_RS05025 (1021168) 1021168..1022178 + 1011 WP_005051459.1 DNA-binding transcriptional regulator AscG -
JMV05_RS05030 (1022327) 1022327..1022854 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12298.98 Da        Isoelectric Point: 9.4924

>T289444 WP_000547562.1 NZ_LR878365:1017176-1017487 [Shigella flexneri 3a]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15397.33 Da        Isoelectric Point: 4.3648

>AT289444 WP_000126288.1 NZ_LR878365:1017484-1017903 [Shigella flexneri 3a]
MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Y4XNA6

Antitoxin

Source ID Structure

References