Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4556604..4557332 Replicon chromosome
Accession NZ_LR778152
Organism Escherichia coli isolate SC477

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag H1D09_RS22030 Protein ID WP_000547555.1
Coordinates 4556604..4556915 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H1D09_RS22035 Protein ID WP_180344733.1
Coordinates 4556916..4557332 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D09_RS22000 4551748..4553457 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
H1D09_RS22005 4553467..4554009 + 543 WP_000493799.1 formate hydrogenlyase subunit HycF -
H1D09_RS22010 4554009..4554776 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
H1D09_RS22015 4554773..4555183 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
H1D09_RS22020 4555176..4555643 + 468 WP_000132953.1 hydrogenase maturation peptidase HycI -
H1D09_RS22025 4555686..4556441 + 756 WP_175058766.1 hypothetical protein -
H1D09_RS22030 4556604..4556915 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
H1D09_RS22035 4556916..4557332 + 417 WP_180344733.1 helix-turn-helix domain-containing protein Antitoxin
H1D09_RS22040 4557424..4557852 - 429 WP_000536064.1 hypothetical protein -
H1D09_RS22045 4558237..4558764 + 528 WP_001078777.1 electron transport protein HydN -
H1D09_RS22050 4558917..4561169 + 2253 WP_175058730.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-137)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T289300 WP_000547555.1 NZ_LR778152:4556604-4556915 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15433.41 Da        Isoelectric Point: 4.3961

>AT289300 WP_180344733.1 NZ_LR778152:4556916-4557332 [Escherichia coli]
MTNAERAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPVG
VALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References