Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2369538..2370232 Replicon chromosome
Accession NZ_LR778152
Organism Escherichia coli isolate SC477

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag H1D09_RS11640 Protein ID WP_001263500.1
Coordinates 2369538..2369936 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag H1D09_RS11645 Protein ID WP_000554758.1
Coordinates 2369939..2370232 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D09_RS11615 2364847..2365305 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
H1D09_RS11620 2365566..2367023 + 1458 WP_175058567.1 cytosol nonspecific dipeptidase -
H1D09_RS11625 2367080..2367694 - 615 WP_000602123.1 peptide chain release factor H -
H1D09_RS11630 2367691..2368830 - 1140 WP_180344730.1 RNA ligase RtcB family protein -
H1D09_RS11635 2369076..2369528 - 453 WP_089573738.1 GNAT family N-acetyltransferase -
H1D09_RS11640 2369538..2369936 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H1D09_RS11645 2369939..2370232 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H1D09_RS11650 2370284..2371339 - 1056 WP_001226166.1 DNA polymerase IV -
H1D09_RS11655 2371410..2372333 - 924 WP_060578024.1 putative lateral flagellar export/assembly protein LafU -
H1D09_RS11660 2372336..2373199 - 864 WP_001169518.1 flagellar motor stator protein MotA -
H1D09_RS11665 2373212..2373928 - 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
H1D09_RS11670 2373948..2374415 - 468 WP_000725261.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T289288 WP_001263500.1 NZ_LR778152:c2369936-2369538 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT289288 WP_000554758.1 NZ_LR778152:c2370232-2369939 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References