Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1135910..1136472 Replicon chromosome
Accession NZ_LR778152
Organism Escherichia coli isolate SC477

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag H1D09_RS05440 Protein ID WP_000605675.1
Coordinates 1135910..1136188 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag H1D09_RS05445 Protein ID WP_000781370.1
Coordinates 1136188..1136472 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D09_RS05415 1131496..1131927 - 432 WP_000152305.1 peroxiredoxin OsmC -
H1D09_RS05420 1132273..1132488 + 216 WP_000495766.1 biofilm-dependent modulation protein -
H1D09_RS05425 1132590..1132727 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
H1D09_RS05430 1132883..1134580 + 1698 WP_000433462.1 malate dehydrogenase -
H1D09_RS05435 1134714..1135724 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
H1D09_RS05440 1135910..1136188 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H1D09_RS05445 1136188..1136472 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
H1D09_RS05450 1136701..1137354 - 654 WP_175058448.1 formate dehydrogenase-N subunit gamma -
H1D09_RS05455 1137347..1138231 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
H1D09_RS05460 1138244..1141291 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T289285 WP_000605675.1 NZ_LR778152:1135910-1136188 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT289285 WP_000781370.1 NZ_LR778152:1136188-1136472 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References