Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 630014..630516 Replicon chromosome
Accession NZ_LR778152
Organism Escherichia coli isolate SC477

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H1D09_RS02770 Protein ID WP_000767829.1
Coordinates 630262..630516 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H1D09_RS02765 Protein ID WP_001259255.1
Coordinates 630014..630265 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D09_RS02740 625191..626259 - 1069 Protein_535 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H1D09_RS02745 626259..627329 - 1071 WP_175058404.1 histidinol-phosphate transaminase -
H1D09_RS02750 627326..628630 - 1305 WP_175058405.1 histidinol dehydrogenase -
H1D09_RS02755 628636..629535 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H1D09_RS02760 629681..629731 - 51 WP_001364200.1 his operon leader peptide -
H1D09_RS02765 630014..630265 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H1D09_RS02770 630262..630516 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H1D09_RS02775 630599..631423 + 825 WP_000754737.1 SDR family oxidoreductase -
H1D09_RS02780 631469..632398 + 930 WP_000803366.1 LysR family transcriptional regulator -
H1D09_RS02785 632613..632675 + 63 WP_010723108.1 membrane protein YoeI -
H1D09_RS02790 632665..634023 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T289280 WP_000767829.1 NZ_LR778152:630262-630516 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T289280 NZ_LR778152:630262-630516 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAGCCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT289280 WP_001259255.1 NZ_LR778152:630014-630265 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT289280 NZ_LR778152:630014-630265 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References