Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 559342..560141 Replicon chromosome
Accession NZ_LR778148
Organism Escherichia coli isolate SC475

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag H1C74_RS02725 Protein ID WP_000347267.1
Coordinates 559677..560141 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H1C74_RS02720 Protein ID WP_001307405.1
Coordinates 559342..559677 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1C74_RS02705 555127..555897 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
H1C74_RS02710 555913..557247 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H1C74_RS02715 557622..559193 + 1572 WP_001273741.1 galactarate dehydratase -
H1C74_RS02720 559342..559677 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H1C74_RS02725 559677..560141 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H1C74_RS02730 560196..561005 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H1C74_RS02735 561254..562534 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H1C74_RS02740 562557..563030 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H1C74_RS02745 563041..563820 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H1C74_RS02750 563810..564688 + 879 WP_001380079.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H1C74_RS02755 564706..565140 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 548686..560141 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T289212 WP_000347267.1 NZ_LR778148:559677-560141 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT289212 WP_001307405.1 NZ_LR778148:559342-559677 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References