Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3231387..3232114 Replicon chromosome
Accession NZ_LR778142
Organism Escherichia coli isolate SC434

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag H1D10_RS15770 Protein ID WP_000547555.1
Coordinates 3231803..3232114 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H1D10_RS15765 Protein ID WP_175058728.1
Coordinates 3231387..3231806 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D10_RS15750 3227550..3229802 - 2253 WP_175058730.1 carbamoyltransferase HypF -
H1D10_RS15755 3229955..3230482 - 528 WP_001078777.1 electron transport protein HydN -
H1D10_RS15760 3230867..3231295 + 429 WP_000536064.1 hypothetical protein -
H1D10_RS15765 3231387..3231806 - 420 WP_175058728.1 helix-turn-helix domain-containing protein Antitoxin
H1D10_RS15770 3231803..3232114 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
H1D10_RS15775 3232277..3233032 - 756 WP_175058766.1 hypothetical protein -
H1D10_RS15780 3233075..3233542 - 468 WP_000132953.1 hydrogenase maturation peptidase HycI -
H1D10_RS15785 3233535..3233945 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
H1D10_RS15790 3233942..3234709 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
H1D10_RS15795 3234709..3235251 - 543 WP_000493799.1 formate hydrogenlyase subunit HycF -
H1D10_RS15800 3235261..3236970 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T289122 WP_000547555.1 NZ_LR778142:c3232114-3231803 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15504.48 Da        Isoelectric Point: 4.3961

>AT289122 WP_175058728.1 NZ_LR778142:c3231806-3231387 [Escherichia coli]
MTANAERAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References