Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1993967..1994529 Replicon chromosome
Accession NZ_LR778142
Organism Escherichia coli isolate SC434

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag H1D10_RS09790 Protein ID WP_000605675.1
Coordinates 1994251..1994529 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag H1D10_RS09785 Protein ID WP_000781370.1
Coordinates 1993967..1994251 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D10_RS09770 1989148..1992195 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
H1D10_RS09775 1992208..1993092 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
H1D10_RS09780 1993085..1993738 + 654 WP_175058448.1 formate dehydrogenase-N subunit gamma -
H1D10_RS09785 1993967..1994251 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
H1D10_RS09790 1994251..1994529 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H1D10_RS09795 1994715..1995725 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
H1D10_RS09800 1995859..1997556 - 1698 WP_000433462.1 malate dehydrogenase -
H1D10_RS09805 1997712..1997849 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
H1D10_RS09810 1997951..1998166 - 216 WP_000495766.1 biofilm-dependent modulation protein -
H1D10_RS09815 1998512..1998943 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T289116 WP_000605675.1 NZ_LR778142:c1994529-1994251 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT289116 WP_000781370.1 NZ_LR778142:c1994251-1993967 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References