Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 760219..760913 Replicon chromosome
Accession NZ_LR778142
Organism Escherichia coli isolate SC434

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag H1D10_RS03580 Protein ID WP_001263500.1
Coordinates 760515..760913 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag H1D10_RS03575 Protein ID WP_000554758.1
Coordinates 760219..760512 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D10_RS03550 756036..756503 + 468 WP_000725261.1 flagellar basal body-associated FliL family protein -
H1D10_RS03555 756523..757239 + 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
H1D10_RS03560 757252..758115 + 864 WP_001169518.1 flagellar motor stator protein MotA -
H1D10_RS03565 758118..759041 + 924 WP_060578024.1 putative lateral flagellar export/assembly protein LafU -
H1D10_RS03570 759112..760167 + 1056 WP_001226166.1 DNA polymerase IV -
H1D10_RS03575 760219..760512 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H1D10_RS03580 760515..760913 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H1D10_RS03585 760923..761375 + 453 WP_089573738.1 GNAT family N-acetyltransferase -
H1D10_RS03590 761621..762760 + 1140 WP_180344730.1 RNA ligase RtcB family protein -
H1D10_RS03595 762757..763371 + 615 WP_000602123.1 peptide chain release factor H -
H1D10_RS03600 763428..764885 - 1458 WP_175058567.1 cytosol nonspecific dipeptidase -
H1D10_RS03605 765146..765604 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T289113 WP_001263500.1 NZ_LR778142:760515-760913 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT289113 WP_000554758.1 NZ_LR778142:760219-760512 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References