Detailed information of TA system TA289016

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 94126..94551
• Replicon: 1 day old chicken plasmid pAPEC2908
• Accession: NZ_LR740777
• Organism: Escherichia coli strain BEN2908 isolate

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: APEC2908_RS25215
• Protein ID: WP_001332444.1
• Coordinates: 94126..94287 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	94340..94551 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APEC2908_RS25185	90431..90652	-	222	WP_001278690.1	conjugal transfer protein TraR	-
APEC2908_RS25190	90787..91302	-	516	WP_000809834.1	type IV conjugative transfer system lipoprotein TraV	-
APEC2908_RS25195	91299..91550	-	252	WP_001038351.1	conjugal transfer protein TrbG	-
APEC2908_RS25200	91594..91980	+	387	Protein_89	transglycosylase SLT domain-containing protein	-
APEC2908_RS25205	92276..93097	-	822	WP_001234485.1	DUF945 domain-containing protein	-
APEC2908_RS25210	93276..93888	+	613	Protein_91	hypothetical protein	-
APEC2908_RS25215	94126..94287	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	94340..94551	-	212	NuclAT_0	-	Antitoxin
-	94340..94551	-	212	NuclAT_0	-	Antitoxin
-	94340..94551	-	212	NuclAT_0	-	Antitoxin
-	94340..94551	-	212	NuclAT_0	-	Antitoxin
APEC2908_RS25220	94562..95281	-	720	WP_001276275.1	plasmid SOS inhibition protein A	-
APEC2908_RS25225	95278..95712	-	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
APEC2908_RS25230	95767..97725	-	1959	Protein_95	ParB/RepB/Spo0J family partition protein	-
APEC2908_RS25235	97784..98017	-	234	WP_001209608.1	DUF905 domain-containing protein	-
APEC2908_RS25240	98080..98619	-	540	WP_161476342.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..152011	152011
	-	flank	IS/Tn	-	-	99418..99921	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T289016 WP_001332444.1 NZ_LR740777:c94287-94126 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

Antitoxin

Nucleotide

• Length: 212 bp

>AT289016 NZ_LR740777:c94551-94340 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin