Detailed information of TA system TA288989

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51993..52410
• Replicon: plasmid pbAPEC5202
• Accession: NZ_LR740760
• Organism: Escherichia coli strain BEN5202 isolate laying hen 51 weeks old

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: APEC5202_RS26335
• Protein ID: WP_001312861.1
• Coordinates: 52252..52410 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	51993..52187 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APEC5202_RS26650	47252..47635	+	384	WP_162542035.1	hypothetical protein	-
APEC5202_RS26310	48012..48464	+	453	WP_103530050.1	single-stranded DNA-binding protein	-
APEC5202_RS26315	48526..48759	+	234	WP_000006030.1	DUF905 family protein	-
APEC5202_RS26320	48818..50776	+	1959	WP_141088311.1	ParB/RepB/Spo0J family partition protein	-
APEC5202_RS26325	50831..51265	+	435	WP_123004679.1	conjugation system SOS inhibitor PsiB	-
APEC5202_RS26330	51262..51981	+	720	WP_029490503.1	plasmid SOS inhibition protein A	-
-	51993..52187	+	195	NuclAT_0	-	Antitoxin
-	51993..52187	+	195	NuclAT_0	-	Antitoxin
-	51993..52187	+	195	NuclAT_0	-	Antitoxin
-	51993..52187	+	195	NuclAT_0	-	Antitoxin
APEC5202_RS26335	52252..52410	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
APEC5202_RS26345	53334..53621	+	288	WP_000107526.1	hypothetical protein	-
APEC5202_RS26350	53740..54561	+	822	WP_029490175.1	DUF945 domain-containing protein	-
APEC5202_RS26355	54857..55504	-	648	WP_141088316.1	transglycosylase SLT domain-containing protein	-
APEC5202_RS26360	55790..56173	+	384	WP_001151564.1	relaxosome protein TraM	-
APEC5202_RS26365	56367..57059	+	693	WP_103530060.1	PAS domain-containing protein	-
APEC5202_RS26370	57164..57391	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..92214	92214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T288989 WP_001312861.1 NZ_LR740760:52252-52410 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 195 bp

>AT288989 NZ_LR740760:51993-52187 [Escherichia coli]
TCACACGGATTACCCGTAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin