Detailed information of TA system TA288983

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 158365..158790
• Replicon: plasmid paAPEC5202
• Accession: NZ_LR740759
• Organism: Escherichia coli strain BEN5202 isolate laying hen 51 weeks old

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: APEC5202_RS25655
• Protein ID: WP_001332444.1
• Coordinates: 158629..158790 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	158365..158576 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APEC5202_RS25630	154339..154836	+	498	WP_050427786.1	single-stranded DNA-binding protein	-
APEC5202_RS25635	154899..155132	+	234	WP_001209608.1	DUF905 domain-containing protein	-
APEC5202_RS25640	155191..157149	+	1959	Protein_150	ParB/RepB/Spo0J family partition protein	-
APEC5202_RS25645	157204..157638	+	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
APEC5202_RS25650	157635..158354	+	720	WP_001276275.1	plasmid SOS inhibition protein A	-
-	158365..158576	+	212	NuclAT_0	-	Antitoxin
-	158365..158576	+	212	NuclAT_0	-	Antitoxin
-	158365..158576	+	212	NuclAT_0	-	Antitoxin
-	158365..158576	+	212	NuclAT_0	-	Antitoxin
APEC5202_RS25655	158629..158790	+	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
APEC5202_RS25660	159028..159640	-	613	Protein_154	hypothetical protein	-
APEC5202_RS25665	159819..160640	+	822	WP_032174246.1	DUF945 domain-containing protein	-
APEC5202_RS25670	160936..161526	-	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
APEC5202_RS25675	161860..162243	+	384	WP_000124981.1	relaxosome protein TraM	-
APEC5202_RS25680	162433..163119	+	687	WP_000332490.1	PAS domain-containing protein	-
APEC5202_RS25685	163213..163440	+	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..223939	223939
	-	flank	IS/Tn	-	-	152990..153367	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T288983 WP_001332444.1 NZ_LR740759:158629-158790 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

Antitoxin

Nucleotide

• Length: 212 bp

>AT288983 NZ_LR740759:158365-158576 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin