288952

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 284019..284713 Replicon chromosome
Accession NZ_LR740758
Organism Escherichia coli strain BEN5202 isolate laying hen 51 weeks old

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag APEC5202_RS01305 Protein ID WP_001263500.1
Coordinates 284315..284713 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag APEC5202_RS01300 Protein ID WP_000554758.1
Coordinates 284019..284312 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APEC5202_RS01275 279555..280052 + 498 WP_000006241.1 REP-associated tyrosine transposase RayT -
APEC5202_RS01280 280135..280293 - 159 WP_014639450.1 hypothetical protein -
APEC5202_RS01285 280372..282084 - 1713 Protein_252 flagellar biosynthesis protein FlhA -
APEC5202_RS01290 282056..282841 + 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
APEC5202_RS01295 282912..283967 + 1056 WP_001226168.1 DNA polymerase IV -
APEC5202_RS01300 284019..284312 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
APEC5202_RS01305 284315..284713 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
APEC5202_RS01310 284723..285175 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
APEC5202_RS01315 285365..286504 + 1140 WP_000521577.1 RNA ligase RtcB family protein -
APEC5202_RS01320 286501..287115 + 615 WP_000602123.1 peptide chain release factor H -
APEC5202_RS01325 287172..288629 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
APEC5202_RS01330 288890..289348 + 459 WP_001291988.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 264495..285175 20680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T288952 WP_001263500.1 NZ_LR740758:284315-284713 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT288952 WP_000554758.1 NZ_LR740758:284019-284312 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References