Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2173524..2174392 Replicon chromosome
Accession NC_020245
Organism Mycobacterium bovis BCG str. Korea 1168P

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag K60_RS10075 Protein ID WP_010886136.1
Coordinates 2173524..2173901 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag K60_RS10080 Protein ID WP_003409886.1
Coordinates 2173943..2174392 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K60_RS10025 2169313..2169765 - 453 WP_010950636.1 lipoprotein -
K60_RS10030 2169829..2170230 + 402 WP_003409869.1 hypothetical protein -
K60_RS10035 2170223..2170405 - 183 WP_003409870.1 hypothetical protein -
K60_RS10040 2170519..2170869 - 351 WP_003409871.1 hypothetical protein -
K60_RS10045 2170880..2171782 - 903 WP_003409874.1 hypothetical protein -
K60_RS10050 2171803..2171994 - 192 WP_003409876.1 hypothetical protein -
K60_RS10055 2171995..2172291 - 297 WP_003409877.1 hypothetical protein -
K60_RS10060 2172531..2172746 + 216 WP_003409878.1 antitoxin -
K60_RS10065 2172743..2173054 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
K60_RS22300 2173028..2173549 - 522 WP_010950637.1 hypothetical protein -
K60_RS10075 2173524..2173901 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
K60_RS10080 2173943..2174392 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
K60_RS10085 2174389..2174934 + 546 WP_003409891.1 SecB-like chaperone -
K60_RS10090 2174823..2175437 - 615 WP_003901296.1 hypothetical protein -
K60_RS10095 2175486..2175782 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
K60_RS10100 2175779..2176030 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
K60_RS10105 2176017..2176511 + 495 WP_003899099.1 hypothetical protein -
K60_RS10110 2176671..2177078 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
K60_RS10115 2177082..2177354 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
K60_RS10120 2177387..2178607 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T28895 WP_010886136.1 NC_020245:2173524-2173901 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T28895 NC_020245:2173524-2173901 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT28895 WP_003409886.1 NC_020245:2173943-2174392 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT28895 NC_020245:2173943-2174392 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References