Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3520488..3521158 Replicon chromosome
Accession NZ_LR699570
Organism Mycobacterium tuberculosis variant bovis strain Mb3601 isolate 14Z005608

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag MBS3601_RS16435 Protein ID WP_003899954.1
Coordinates 3520488..3520832 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag MBS3601_RS16440 Protein ID WP_003899955.1
Coordinates 3520829..3521158 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBS3601_RS16405 3515561..3516421 + 861 WP_003416628.1 alpha/beta hydrolase -
MBS3601_RS16410 3516396..3516911 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
MBS3601_RS16415 3517151..3517444 + 294 WP_003416635.1 hypothetical protein -
MBS3601_RS16420 3517732..3519021 + 1290 WP_003416640.1 ATP-binding protein -
MBS3601_RS16425 3519368..3519802 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
MBS3601_RS16430 3519805..3520257 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MBS3601_RS16435 3520488..3520832 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MBS3601_RS16440 3520829..3521158 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
MBS3601_RS16445 3521696..3522043 + 348 WP_003899956.1 DUF2384 domain-containing protein -
MBS3601_RS16450 3522040..3522660 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
MBS3601_RS16455 3522820..3524085 - 1266 WP_003909837.1 hypothetical protein -
MBS3601_RS16460 3524253..3524462 + 210 WP_003416778.1 hypothetical protein -
MBS3601_RS16465 3524709..3525743 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T288861 WP_003899954.1 NZ_LR699570:3520488-3520832 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT288861 WP_003899955.1 NZ_LR699570:3520829-3521158 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References