Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2255959..2256878 Replicon chromosome
Accession NZ_LR699570
Organism Mycobacterium tuberculosis variant bovis strain Mb3601 isolate 14Z005608

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag MBS3601_RS10455 Protein ID WP_003900449.1
Coordinates 2256273..2256878 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag MBS3601_RS10450 Protein ID WP_003410124.1
Coordinates 2255959..2256264 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBS3601_RS10420 2251222..2251797 + 576 WP_003410100.1 hypothetical protein -
MBS3601_RS10425 2251794..2252834 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
MBS3601_RS10430 2252944..2254205 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
MBS3601_RS10435 2254434..2255153 + 720 WP_010950643.1 DUF433 domain-containing protein -
MBS3601_RS10440 2255143..2255559 + 417 WP_003410114.1 hypothetical protein -
MBS3601_RS10445 2255575..2255874 - 300 WP_003410120.1 hypothetical protein -
MBS3601_RS10450 2255959..2256264 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
MBS3601_RS10455 2256273..2256878 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MBS3601_RS10460 2256903..2257262 - 360 WP_003410131.1 hypothetical protein -
MBS3601_RS10465 2257422..2258099 - 678 WP_003900450.1 hypothetical protein -
MBS3601_RS10470 2258051..2258287 - 237 WP_003410133.1 hypothetical protein -
MBS3601_RS10475 2258494..2259390 - 897 WP_011799238.1 hypothetical protein -
MBS3601_RS10480 2259574..2260899 - 1326 Protein_2071 helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T288844 WP_003900449.1 NZ_LR699570:c2256878-2256273 [Mycobacterium tuberculosis variant bovis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT288844 WP_003410124.1 NZ_LR699570:c2256264-2255959 [Mycobacterium tuberculosis variant bovis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References