Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2202233..2203101 Replicon chromosome
Accession NZ_LR699570
Organism Mycobacterium tuberculosis variant bovis strain Mb3601 isolate 14Z005608

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag MBS3601_RS10140 Protein ID WP_010886136.1
Coordinates 2202233..2202610 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag MBS3601_RS10145 Protein ID WP_003409886.1
Coordinates 2202652..2203101 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBS3601_RS10090 2198022..2198474 - 453 WP_010950636.1 lipoprotein -
MBS3601_RS10095 2198538..2198939 + 402 WP_003409869.1 hypothetical protein -
MBS3601_RS10100 2198932..2199114 - 183 WP_003409870.1 hypothetical protein -
MBS3601_RS10105 2199228..2199578 - 351 WP_003409871.1 hypothetical protein -
MBS3601_RS10110 2199589..2200491 - 903 WP_003409874.1 hypothetical protein -
MBS3601_RS10115 2200512..2200703 - 192 WP_003409876.1 hypothetical protein -
MBS3601_RS10120 2200704..2201000 - 297 WP_003409877.1 hypothetical protein -
MBS3601_RS10125 2201240..2201455 + 216 WP_003409878.1 antitoxin -
MBS3601_RS10130 2201452..2201763 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
MBS3601_RS10135 2201737..2202258 - 522 WP_010950637.1 hypothetical protein -
MBS3601_RS10140 2202233..2202610 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
MBS3601_RS10145 2202652..2203101 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MBS3601_RS10150 2203098..2203643 + 546 WP_003409891.1 SecB-like chaperone -
MBS3601_RS10155 2203532..2204146 - 615 WP_003901296.1 hypothetical protein -
MBS3601_RS10160 2204195..2204491 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
MBS3601_RS10165 2204488..2204739 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
MBS3601_RS10170 2204726..2205220 + 495 WP_003899099.1 hypothetical protein -
MBS3601_RS10175 2205380..2205787 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
MBS3601_RS10180 2205791..2206063 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MBS3601_RS10185 2206096..2207316 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T288838 WP_010886136.1 NZ_LR699570:2202233-2202610 [Mycobacterium tuberculosis variant bovis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT288838 WP_003409886.1 NZ_LR699570:2202652-2203101 [Mycobacterium tuberculosis variant bovis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References