Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 5363210..5363805 Replicon chromosome
Accession NZ_LR657304
Organism Pseudomonas aeruginosa PAK

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag PAKAF_RS24755 Protein ID WP_003113526.1
Coordinates 5363527..5363805 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PAKAF_RS24750 Protein ID WP_003113527.1
Coordinates 5363210..5363515 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAKAF_RS24720 5358880..5359170 - 291 WP_016253916.1 DUF5447 family protein -
PAKAF_RS24725 5359382..5359654 - 273 WP_003115921.1 hypothetical protein -
PAKAF_RS24730 5359764..5360030 + 267 WP_016253917.1 hypothetical protein -
PAKAF_RS24735 5360086..5360421 + 336 WP_031759015.1 hypothetical protein -
PAKAF_RS24740 5360456..5361601 + 1146 WP_016253918.1 DUF262 domain-containing protein -
PAKAF_RS24745 5361604..5362371 + 768 WP_016253919.1 hypothetical protein -
PAKAF_RS24750 5363210..5363515 - 306 WP_003113527.1 HigA family addiction module antidote protein Antitoxin
PAKAF_RS24755 5363527..5363805 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PAKAF_RS24760 5364134..5366362 + 2229 WP_016253920.1 TonB-dependent receptor -
PAKAF_RS24765 5366432..5367076 - 645 WP_016253921.1 carbonate dehydratase -
PAKAF_RS24770 5367138..5368376 - 1239 WP_016253922.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T288662 WP_003113526.1 NZ_LR657304:c5363805-5363527 [Pseudomonas aeruginosa PAK]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT288662 WP_003113527.1 NZ_LR657304:c5363515-5363210 [Pseudomonas aeruginosa PAK]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References