Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3307463..3308262 Replicon chromosome
Accession NZ_LR595691
Organism Escherichia coli isolate EcMAD1

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag ECMAD3_RS15970 Protein ID WP_000347251.1
Coordinates 3307798..3308262 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag ECMAD3_RS15965 Protein ID WP_001307405.1
Coordinates 3307463..3307798 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECMAD3_RS15950 3303248..3304018 - 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -
ECMAD3_RS15955 3304034..3305368 - 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
ECMAD3_RS15960 3305743..3307314 + 1572 WP_001273761.1 galactarate dehydratase -
ECMAD3_RS15965 3307463..3307798 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
ECMAD3_RS15970 3307798..3308262 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
ECMAD3_RS15975 3308317..3309126 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
ECMAD3_RS15980 3309375..3310655 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
ECMAD3_RS15985 3310678..3311151 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
ECMAD3_RS15990 3311162..3311941 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
ECMAD3_RS15995 3311931..3312809 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
ECMAD3_RS16000 3312827..3313261 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3296588..3308262 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T288612 WP_000347251.1 NZ_LR595691:3307798-3308262 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT288612 WP_001307405.1 NZ_LR595691:3307463-3307798 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References