Detailed information of TA system TA028853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4688251..4688981
• Replicon: chromosome
• Accession: NC_020211
• Organism: Serratia marcescens WW4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: SMWW4_RS21890
• Protein ID: WP_004937033.1
• Coordinates: 4688251..4688562 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SMWW4_RS21895
• Protein ID: WP_015379178.1
• Coordinates: 4688559..4688981 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMWW4_RS21860	4683566..4684291	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
SMWW4_RS21865	4684344..4685777	+	1434	WP_004937039.1	RNA polymerase factor sigma-54	-
SMWW4_RS21870	4685801..4686088	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
SMWW4_RS21875	4686244..4686705	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
SMWW4_RS21880	4686833..4687687	+	855	WP_015379177.1	RNase adapter RapZ	-
SMWW4_RS21885	4687684..4687956	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
SMWW4_RS21890	4688251..4688562	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
SMWW4_RS21895	4688559..4688981	+	423	WP_015379178.1	helix-turn-helix domain-containing protein	Antitoxin
SMWW4_RS21900	4688985..4690901	-	1917	WP_015379179.1	BglG family transcription antiterminator	-
SMWW4_RS21905	4690962..4692176	-	1215	WP_015379180.1	lactonase family protein	-
SMWW4_RS21910	4692391..4693131	-	741	WP_004937022.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T28853 WP_004937033.1 NC_020211:4688251-4688562 [Serratia marcescens WW4]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T28853 NC_020211:4688251-4688562 [Serratia marcescens WW4]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT28853 WP_015379178.1 NC_020211:4688559-4688981 [Serratia marcescens WW4]
MITDAIKAADALLRAVPLLQGSRAQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT28853 NC_020211:4688559-4688981 [Serratia marcescens WW4]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Toxin

• AlphaFold DB: A0A8B4GR63