Detailed information of TA system TA028852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4632185..4632813
• Replicon: chromosome
• Accession: NC_020211
• Organism: Serratia marcescens WW4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: SMWW4_RS21590
• Protein ID: WP_041922289.1
• Coordinates: 4632185..4632475 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B4GQY7
• Locus tag: SMWW4_RS21595
• Protein ID: WP_015379150.1
• Coordinates: 4632490..4632813 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMWW4_RS21580	4628599..4630620	+	2022	WP_015379147.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
SMWW4_RS21585	4630815..4631954	-	1140	WP_015379148.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
SMWW4_RS21590	4632185..4632475	+	291	WP_041922289.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SMWW4_RS21595	4632490..4632813	+	324	WP_015379150.1	HigA family addiction module antidote protein	Antitoxin
SMWW4_RS21600	4632806..4633318	+	513	WP_015379151.1	M48 family metallopeptidase	-
SMWW4_RS21605	4633368..4634348	+	981	WP_004937152.1	Gfo/Idh/MocA family oxidoreductase	-
SMWW4_RS21610	4634350..4635081	+	732	WP_015379152.1	glutamine amidotransferase	-
SMWW4_RS21615	4635337..4636302	+	966	WP_049241556.1	TerC family protein	-
SMWW4_RS21620	4636565..4637800	+	1236	WP_015379154.1	serine/threonine transporter SstT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11608.26 Da
• Isoelectric Point: 10.0176

>T28852 WP_041922289.1 NC_020211:4632185-4632475 [Serratia marcescens WW4]
MIKSFRDRYLEQFYLEGKRSRLIPGVLERQLARKLDILAAAQQERDLHHPTGNYYKRLSGPFQGWSSLHVNMHWRLMFQW
RHDAAEKIYLDPHQDT

Nucleotide

• Length: 291 bp

>T28852 NC_020211:4632185-4632475 [Serratia marcescens WW4]
ATGATTAAATCTTTCCGCGATCGCTATCTGGAGCAGTTCTATCTGGAAGGCAAACGCAGCCGTCTGATCCCCGGCGTGCT
CGAGCGGCAGTTGGCCAGAAAGCTCGATATATTGGCCGCCGCGCAGCAAGAGCGTGATTTGCATCACCCCACGGGTAACT
ATTACAAGCGGCTATCCGGGCCATTTCAGGGATGGTCAAGTCTGCACGTCAATATGCACTGGCGGCTGATGTTCCAGTGG
CGGCATGATGCCGCTGAAAAAATTTACCTCGATCCTCACCAAGATACCTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12141.78 Da
• Isoelectric Point: 6.2285

>AT28852 WP_015379150.1 NC_020211:4632490-4632813 [Serratia marcescens WW4]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Nucleotide

• Length: 324 bp

>AT28852 NC_020211:4632490-4632813 [Serratia marcescens WW4]
ATGTTGGAAACCACGACTCGCGAACCCACCACGGTGGGGGAAGTGCTGCAGGAAGAGTATTTAACGCCGTTGGGCATCGG
CCAGGAAGAACTGGCGAAGGTACTGGGCGTCACCCGTGCCACTGTTAACCGCCTGTGCAACGGGCATCGACGCATGAGCG
TTGCCGAGGCCAACCTGTTTGCCGACCTGTTCGAAACGACCGCCGACTTTTGGCTAAACCTGCAGGCGGCACACGATCGC
TGGGAAGCCAGACAGGCCGATGCGCAACGTGTGCGGCTGCGGCCAATCAAAGAAATACTGGGACGCAATATCACTCATGC
ATGA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GQY7