Detailed information of TA system TA288516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1951203..1951863
• Replicon: chromosome
• Accession: NZ_LR594038
• Organism: Klebsiella grimontii strain NCTC9146 substr. serovar casular

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FGL39_RS09305
• Protein ID: WP_049089248.1
• Coordinates: 1951203..1951556 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A9E1DQ94
• Locus tag: FGL39_RS09310
• Protein ID: WP_004132739.1
• Coordinates: 1951561..1951863 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGL39_RS09280	1946587..1947354	-	768	WP_004132727.1	molybdopterin-dependent oxidoreductase	-
FGL39_RS09285	1947344..1947952	-	609	WP_004132729.1	cytochrome b/b6 domain-containing protein	-
FGL39_RS09290	1947967..1948593	-	627	WP_004132731.1	hypothetical protein	-
FGL39_RS09295	1948733..1949401	+	669	WP_004132733.1	heavy metal response regulator transcription factor	-
FGL39_RS09300	1949401..1950819	+	1419	WP_049085817.1	heavy metal sensor histidine kinase	-
FGL39_RS09305	1951203..1951556	+	354	WP_049089248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FGL39_RS09310	1951561..1951863	+	303	WP_004132739.1	XRE family transcriptional regulator	Antitoxin
FGL39_RS09315	1951954..1952127	+	174	WP_101868488.1	helix-turn-helix domain-containing protein	-
FGL39_RS09320	1952250..1952453	+	204	Protein_1807	hypothetical protein	-
FGL39_RS09325	1952454..1952787	+	334	Protein_1808	type II toxin-antitoxin system PemK/MazF family toxin	-
FGL39_RS09330	1953126..1953263	+	138	Protein_1809	patatin	-
FGL39_RS09335	1953404..1954285	-	882	WP_064793685.1	hypothetical protein	-
FGL39_RS09340	1954694..1954942	+	249	WP_138110103.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13639.64 Da
• Isoelectric Point: 9.8874

>T288516 WP_049089248.1 NZ_LR594038:1951203-1951556 [Klebsiella grimontii]
VWMIKTTDTFERWFTSLNDTDRARVLAALLVLREKGPGLSRPYADTLRGSRYSDMKELRIQSRGEPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMLPVAEREFTNWLKTFKEKE

Nucleotide

• Length: 354 bp

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11072.83 Da
• Isoelectric Point: 5.7424

>AT288516 WP_004132739.1 NZ_LR594038:1951561-1951863 [Klebsiella grimontii]
MGRTLEQLMADEKPEVVADARAMAADILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFEL

Nucleotide

• Length: 303 bp