Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1172435..1173192 Replicon chromosome
Accession NZ_LR536431
Organism Escherichia coli isolate f9610206-5e81-11e8-bf7f-3c4a9275d6c8

Toxin (Protein)


Gene name higB Uniprot ID A0A377MUG9
Locus tag E4Z82_RS05790 Protein ID WP_000266200.1
Coordinates 1172435..1172776 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E4Z82_RS05795 Protein ID WP_000126296.1
Coordinates 1172773..1173192 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z82_RS05760 1167618..1169327 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
E4Z82_RS05765 1169337..1169879 + 543 WP_000493763.1 formate hydrogenlyase subunit HycF -
E4Z82_RS05770 1169879..1170646 + 768 WP_000067392.1 formate hydrogenlyase subunit HycG -
E4Z82_RS05775 1170643..1171053 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
E4Z82_RS05780 1171046..1171516 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
E4Z82_RS05785 1171541..1172314 + 774 WP_001026444.1 hypothetical protein -
E4Z82_RS05790 1172435..1172776 + 342 WP_000266200.1 type II toxin-antitoxin system HigB family toxin Toxin
E4Z82_RS05795 1172773..1173192 + 420 WP_000126296.1 helix-turn-helix domain-containing protein Antitoxin
E4Z82_RS05800 1173306..1174730 - 1425 WP_000110319.1 6-phospho-beta-glucosidase AscB -
E4Z82_RS05805 1174739..1176196 - 1458 WP_001107847.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
E4Z82_RS05810 1176456..1177466 + 1011 WP_001402444.1 DNA-binding transcriptional regulator AscG -
E4Z82_RS05815 1177615..1178142 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(32-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13733.68 Da        Isoelectric Point: 9.7506

>T288408 WP_000266200.1 NZ_LR536431:1172435-1172776 [Escherichia coli]
MWLFTYYKEVKNIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVI
AYINFVNKRFFVKHITNHAEYDKLTRYYRENKE

Download         Length: 342 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.38 Da        Isoelectric Point: 4.3653

>AT288408 WP_000126296.1 NZ_LR536431:1172773-1173192 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377MUG9

Antitoxin

Source ID Structure

References