Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1827108..1827835 Replicon chromosome
Accession NZ_LR536430
Organism Escherichia coli isolate f974b26a-5e81-11e8-bf7f-3c4a9275d6c8

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag E4Z69_RS09325 Protein ID WP_000550189.1
Coordinates 1827521..1827835 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E4Z69_RS09320 Protein ID WP_000560266.1
Coordinates 1827108..1827524 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z69_RS09310 1822168..1824519 + 2352 WP_000695498.1 alpha-glucosidase -
E4Z69_RS09315 1825045..1827063 + 2019 WP_000121421.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
E4Z69_RS09320 1827108..1827524 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
E4Z69_RS09325 1827521..1827835 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
E4Z69_RS09330 1828120..1829256 - 1137 WP_000018697.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
E4Z69_RS09335 1829342..1829845 + 504 WP_001295542.1 M48 family metallopeptidase -
E4Z69_RS09340 1829922..1830605 + 684 WP_001183040.1 vancomycin high temperature exclusion protein -
E4Z69_RS09345 1830684..1831670 + 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T288391 WP_000550189.1 NZ_LR536430:c1827835-1827521 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT288391 WP_000560266.1 NZ_LR536430:c1827524-1827108 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References