Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1401583..1402310 Replicon chromosome
Accession NZ_LR536430
Organism Escherichia coli isolate f974b26a-5e81-11e8-bf7f-3c4a9275d6c8

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag E4Z69_RS07250 Protein ID WP_000547564.1
Coordinates 1401999..1402310 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E4Z69_RS07245 Protein ID WP_000126294.1
Coordinates 1401583..1402002 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z69_RS07225 1396633..1397160 - 528 WP_001078777.1 electron transport protein HydN -
E4Z69_RS07230 1397309..1398319 - 1011 WP_001402444.1 DNA-binding transcriptional regulator AscG -
E4Z69_RS07235 1398579..1400036 + 1458 WP_001107882.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
E4Z69_RS07240 1400045..1401469 + 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
E4Z69_RS07245 1401583..1402002 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
E4Z69_RS07250 1401999..1402310 - 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
E4Z69_RS07255 1402484..1403245 - 762 WP_001026446.1 hypothetical protein -
E4Z69_RS07260 1403270..1403740 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
E4Z69_RS07265 1403733..1404143 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
E4Z69_RS07270 1404140..1404907 - 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
E4Z69_RS07275 1404907..1405449 - 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
E4Z69_RS07280 1405459..1407168 - 1710 WP_134393430.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T288386 WP_000547564.1 NZ_LR536430:c1402310-1401999 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT288386 WP_000126294.1 NZ_LR536430:c1402002-1401583 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References