Detailed information of TA system TA288377

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 250368..250739
• Replicon: chromosome
• Accession: NZ_LR536430
• Organism: Escherichia coli isolate f974b26a-5e81-11e8-bf7f-3c4a9275d6c8

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: E4Z69_RS01375
• Protein ID: WP_001317028.1
• Coordinates: 250545..250739 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	250368..250546 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4Z69_RS01350	246323..247696	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
E4Z69_RS01355	247825..248760	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
E4Z69_RS01360	248812..250047	-	1236	WP_000040852.1	site-specific integrase	-
E4Z69_RS01365	250049..250264	-	216	WP_000079604.1	excisionase XisR	-
E4Z69_RS01370	250343..250552	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	250368..250546	+	179	NuclAT_0	-	Antitoxin
-	250368..250546	+	179	NuclAT_0	-	Antitoxin
-	250368..250546	+	179	NuclAT_0	-	Antitoxin
-	250368..250546	+	179	NuclAT_0	-	Antitoxin
E4Z69_RS01375	250545..250739	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
E4Z69_RS01380	250796..251605	-	810	WP_000166319.1	recombination protein RecT	-
E4Z69_RS01385	251598..254198	-	2601	WP_001549183.1	exodeoxyribonuclease VIII	-
E4Z69_RS01390	254300..254575	-	276	WP_000632297.1	protein RacC	-
E4Z69_RS01395	254650..254820	-	171	WP_001352098.1	conserved protein, Rac prophage	-
E4Z69_RS01400	254820..255041	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	248812..260063	11251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T288377 WP_001317028.1 NZ_LR536430:c250739-250545 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

Antitoxin

Nucleotide

• Length: 179 bp

>AT288377 NZ_LR536430:250368-250546 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAATTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin