288351

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1314639..1315333 Replicon chromosome
Accession NZ_LR217818
Organism Escherichia coli strain NCTC86

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag E4V11_RS06390 Protein ID WP_001263488.1
Coordinates 1314935..1315333 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag E4V11_RS06385 Protein ID WP_000554758.1
Coordinates 1314639..1314932 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4V11_RS06365 1310271..1310768 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
E4V11_RS06370 1310992..1312704 - 1713 Protein_1227 flagellar biosynthesis protein FlhA -
E4V11_RS06375 1312676..1313461 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
E4V11_RS06380 1313532..1314587 + 1056 WP_001226164.1 DNA polymerase IV -
E4V11_RS06385 1314639..1314932 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
E4V11_RS06390 1314935..1315333 + 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
E4V11_RS06395 1315343..1315795 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
E4V11_RS06400 1316113..1316319 + 207 Protein_1233 RtcB family protein -
E4V11_RS06405 1316315..1316836 + 522 Protein_1234 peptide chain release factor H -
E4V11_RS06410 1316893..1318350 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
E4V11_RS06415 1318611..1319069 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 1319665..1319745 + 81 NuclAT_12 - -
- 1319665..1319745 + 81 NuclAT_12 - -
- 1319665..1319745 + 81 NuclAT_12 - -
- 1319665..1319745 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1300876..1331614 30738


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T288351 WP_001263488.1 NZ_LR217818:1314935-1315333 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT288351 WP_000554758.1 NZ_LR217818:1314639-1314932 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References