Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1308595..1309136 Replicon chromosome
Accession NZ_LR217818
Organism Escherichia coli strain NCTC86

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag E4V11_RS06350 Protein ID WP_000615983.1
Coordinates 1308595..1308873 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag E4V11_RS06355 Protein ID WP_000729703.1
Coordinates 1308876..1309136 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4V11_RS06335 1306177..1306755 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
E4V11_RS06340 1306961..1307728 + 768 WP_000333380.1 class II glutamine amidotransferase -
E4V11_RS06345 1307699..1308439 - 741 WP_001225679.1 murein L,D-transpeptidase -
E4V11_RS06350 1308595..1308873 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
E4V11_RS06355 1308876..1309136 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
E4V11_RS06360 1309346..1310095 + 750 WP_000056849.1 C40 family peptidase -
E4V11_RS06365 1310271..1310768 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
E4V11_RS06370 1310992..1312704 - 1713 Protein_1227 flagellar biosynthesis protein FlhA -
E4V11_RS06375 1312676..1313461 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1300876..1331614 30738


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T288350 WP_000615983.1 NZ_LR217818:c1308873-1308595 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT288350 WP_000729703.1 NZ_LR217818:c1309136-1308876 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References