Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3689367..3690061 Replicon chromosome
Accession NZ_LR213455
Organism Shigella flexneri strain AUSMDU00008332 isolate AUSMDU00008332

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag E0E35_RS19295 Protein ID WP_001263491.1
Coordinates 3689367..3689765 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag E0E35_RS19300 Protein ID WP_000554759.1
Coordinates 3689768..3690061 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0E35_RS19270 (3684706) 3684706..3685164 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
E0E35_RS19275 (3685426) 3685426..3686883 + 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
E0E35_RS19280 (3686940) 3686940..3687554 - 615 Protein_3665 peptide chain release factor H -
E0E35_RS19285 (3687616) 3687616..3688659 - 1044 WP_005053205.1 RNA ligase RtcB family protein -
E0E35_RS19290 (3688905) 3688905..3689357 - 453 WP_001059860.1 GNAT family N-acetyltransferase -
E0E35_RS19295 (3689367) 3689367..3689765 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
E0E35_RS19300 (3689768) 3689768..3690061 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
E0E35_RS19305 (3690113) 3690113..3691168 - 1056 WP_001226172.1 DNA polymerase IV -
E0E35_RS19310 (3691239) 3691239..3692024 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
E0E35_RS19315 (3691996) 3691996..3693708 + 1713 Protein_3672 flagellar biosynthesis protein FlhA -
E0E35_RS19320 (3693925) 3693925..3694422 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrII / gtrB / gtrA 3648243..3696981 48738


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T288279 WP_001263491.1 NZ_LR213455:c3689765-3689367 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT288279 WP_000554759.1 NZ_LR213455:c3690061-3689768 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References