Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3565086..3565704 Replicon chromosome
Accession NZ_LR213455
Organism Shigella flexneri strain AUSMDU00008332 isolate AUSMDU00008332

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E0E35_RS18635 Protein ID WP_001291435.1
Coordinates 3565486..3565704 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag E0E35_RS18630 Protein ID WP_000344797.1
Coordinates 3565086..3565460 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0E35_RS18620 (3560175) 3560175..3561368 + 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
E0E35_RS18625 (3561391) 3561391..3564540 + 3150 WP_001132484.1 efflux RND transporter permease AcrB -
E0E35_RS18630 (3565086) 3565086..3565460 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
E0E35_RS18635 (3565486) 3565486..3565704 + 219 WP_001291435.1 HHA domain-containing protein Toxin
E0E35_RS18640 (3565876) 3565876..3566427 + 552 Protein_3544 maltose O-acetyltransferase -
E0E35_RS18645 (3566543) 3566543..3567013 + 471 WP_000136192.1 YlaC family protein -
E0E35_RS18650 (3567177) 3567177..3568727 + 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E0E35_RS18655 (3568769) 3568769..3569122 - 354 WP_000878140.1 DUF1428 domain-containing protein -
E0E35_RS18665 (3569501) 3569501..3569812 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3569842..3571170 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T288277 WP_001291435.1 NZ_LR213455:3565486-3565704 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT288277 WP_000344797.1 NZ_LR213455:3565086-3565460 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References