Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 928..1463 Replicon plasmid pNB2354_1
Accession NC_020208
Organism Enterococcus faecium NRRL B-2354

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag M7W_RS12685 Protein ID WP_002314366.1
Coordinates 928..1200 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag M7W_RS12690 Protein ID WP_002297422.1
Coordinates 1200..1463 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M7W_RS12675 245..532 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
M7W_RS12680 522..851 - 330 WP_002292678.1 AbrB family transcriptional regulator -
M7W_RS12685 928..1200 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
M7W_RS12690 1200..1463 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
M7W_RS12695 1569..2495 - 927 WP_002328829.1 hypothetical protein -
M7W_RS12700 3146..3826 - 681 Protein_5 IS6-like element IS1216 family transposase -
M7W_RS12705 4195..4419 + 225 WP_002333352.1 response regulator -
M7W_RS12715 4446..5572 + 1127 WP_094850654.1 IS3 family transposase -
M7W_RS14805 5597..5905 + 309 Protein_8 GHKL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ClpL - 1..214319 214319


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T28827 WP_002314366.1 NC_020208:c1200-928 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T28827 NC_020208:c1200-928 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT28827 WP_002297422.1 NC_020208:c1463-1200 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT28827 NC_020208:c1463-1200 [Enterococcus faecium ATCC 8459 = NRRL B-2354]
ATGGCAAAAACTGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References